RNA Biology

E-mail: pavel.kovarik(at)univie.ac.at
Phone: 0043 1 4277 54608
Research Group: http://www.mfpl.ac.at/kovarik.html
Institution: MFPL/UniWien

Full member of the Focus Regulatory RNA (SFB RNA-Reg) since 2015, with students associated to the DK.

PhD students associated to the DK:
Lucy Sneezum
Martina Borroni

For complete list of group members please visit the Kovarik group website.

 

Our research:

Inflammation can be viewed as stereotypical set of reactions to protect cells and entire organisms against environmental insults such as invading pathogens or tissue injuries. One of the key features of inflammatory responses is the precise and multilayered control of their magnitude and duration, which are adjusted such that hyper- or chronic inflammation is avoided yet a protective response is generated. We investigate the regulation of immune homeostasis at the level of transcriptional and posttranscriptional control of gene expression. In the field of posttranscriptional mechanisms we focus on the mRNA-destabilizing protein TTP and how TTP regulates inflammatory mRNA degradation to re-install immune and tissue homeostasis. We employ systems biology approaches combining high content approaches such as PAR-CLIP, RNA-seq and GRO-seq with cellular as well as animal models of infection and inflammation.

 

Publications: (since 2015)

 

Competition of Candida glabrata against Lactobacillus is Hog1 dependent.
Beyer R, Jandric Z, Zutz C, Gregori C, Willinger B, Jacobsen ID, Kovarik P, Strauss J, Schüller C.
Cell Microbiol. 2018 Aug 15:e12943.

The Bicarbonate Transporter SLC4A7 Plays a Key Role in Macrophage Phagosome Acidification.
Sedlyarov V, Eichner R, Girardi E, Essletzbichler P, Goldmann U, Nunes-Hasler P, Srndic I, Moskovskich A, Heinz LX, Kartnig F, Bigenzahn JW, Rebsamen M, Kovarik P, Demaurex N, Superti-Furga G.
Cell Host Microbe. 2018 Jun 13;23(6):766-774.e5.

Natural killer cell-intrinsic type I IFN signaling controls Klebsiella pneumoniae growth during lung infection.
Ivin M, Dumigan A, de Vasconcelos FN, Ebner F, Borroni M, Kavirayani A, Przybyszewska KN, Ingram RJ, Lienenklaus S, Kalinke U, Stoiber D, Bengoechea JA, Kovarik P.
PLoS Pathog. 2017 Nov 7;13(11):e1006696.

The RNA-binding protein tristetraprolin schedules apoptosis of pathogen-engaged neutrophils during bacterial infection.
Ebner F, Sedlyarov V, Tasciyan S, Ivin M, Kratochvill F, Gratz N, Kenner L, Villunger A, Sixt M, Kovarik P.
J Clin Invest. 2017 Jun 1;127(6):2051-2065.

HuR Small-Molecule Inhibitor Elicits Differential Effects in Adenomatosis Polyposis and Colorectal Carcinogenesis.
Lang M, Berry D, Passecker K, Mesteri I, Bhuju S, Ebner F, Sedlyarov V, Evstatiev R, Dammann K, Loy A, Kuzyk O, Kovarik P, Khare V, Beibel M, Roma G, Meisner-Kober N, Gasche C.
Cancer Res. 2017 May 1;77(9):2424-2438.

Posttranscriptional regulation of cytokine expression.
Kovarik P, Ebner F, Sedlyarov V.
Cytokine. 2017 Jan;89:21-26

Type I Interferons in Bacterial Infections: A Balancing Act.
Kovarik P, Castiglia V, Ivin M, Ebner F.
Front Immunol. 2016 Dec 26;7:652.

The Influence of Programmed Cell Death in Myeloid Cells on Host Resilience to Infection with Legionella pneumophila or Streptococcus pyogenes.
Gamradt P, Xu Y, Gratz N, Duncan K, Kobzik L, Högler S, Kovarik P, Decker T, Jamieson AM.
PLoS Pathog. 2016 Dec 14;12(12):e1006032.

Tristetraprolin binding site atlas in the macrophage transcriptome reveals a switch for inflammation resolution.
Sedlyarov V, Fallmann J, Ebner F, Huemer J, Sneezum L, Ivin M, Kreiner K, Tanzer A, Vogl C, Hofacker I, Kovarik P.
Mol Syst Biol. 2016 May 13;12(5):868

Type I Interferon Signaling Prevents IL-1β-Driven Lethal Systemic Hyperinflammation during Invasive Bacterial Infection of Soft Tissue.
Castiglia V, Piersigilli A, Ebner F, Janos M, Goldmann O, Damböck U, Kröger A, Weiss S, Knapp S, Jamieson AM, Kirschning C, Kalinke U, Strobl B, Müller M, Stoiber D, Lienenklaus S, Kovarik P.
Cell Host Microbe. 2016 Mar 9;19(3):375-87.

AREsite2: an enhanced database for the comprehensive investigation of AU/GU/U-rich elements.
Fallmann J, Sedlyarov V, Tanzer A, Kovarik P, Hofacker IL.
Nucleic Acids Res. 2016 Jan 4;44(D1):D90-5.

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.
Kratochvill F, Gratz N, Qualls JE, Van De Velde LA, Chi H, Kovarik P, Murray PJ.
Cancer Res. 2015 Aug 1;75(15):3054-64.

Innate immune response to Streptococcus pyogenes depends on the combined activation of TLR13 and TLR2.
Fieber C, Janos M, Koestler T, Gratz N, Li XD, Castiglia V, Aberle M, Sauert M, Wegner M, Alexopoulou L, Kirschning CJ, Chen ZJ, von Haeseler A, Kovarik P.
PLoS One. 2015 Mar 10;10(3):e0119727.

Promoter occupancy of STAT1 in interferon responses is regulated by processive transcription.
Wiesauer I, Gaumannmüller C, Steinparzer I, Strobl B, Kovarik P.
Mol Cell Biol. 2015 Feb;35(4):716-27.