RNA Biology

E-mail: boris.goerke(a)univie.ac.at
Phone: 0043 1 4277 54603
Research Group: https://www.maxperutzlabs.ac.at/research/research-groups/goerke
Institution: Max Perutz Labs/UniWien

Full member of the Focus Regulatory RNA (SFB RNA-REG) since 2015.
Full member of the DoktoratsKolleg (DK) RNA Biology since 2017.

PhD students within/associated to the DK RNA Biology:
Svetlana Durica
Florian Sikora

DK RNA Biology Alumni & their theses:
Muna Ayesha Khan: “Signal perception and transduction within the GlmY/GlmZ small RNA cascade in E.coli”

For complete list of group members please visit the Görke group website.

 

Signal Transduction and post-transcriptional regulation in model bacteria

In recent years post-transcriptional control of gene expression by small regulatory RNAs (sRNAs) has emerged as a major principle of gene regulation in bacteria. Due to their central regulatory roles in virtually all physiological circuits, sRNA activities must be tightly controlled.  This might occur either at the level of their biogenesis or turn-over. Using the GlmY/GlmZ sRNA circuit of E. coli as a model system we aim to obtain insight into these important questions. In particular, we are interested to (I) unravel the various factors and conditions controlling expression of sRNAs and (II) to identify the components of the machineries specifically controlling the decay of sRNAs.

 

Publications: (since 2015)

Feedback regulation of small RNA processing by the cleavage product.
Durica-Mitic S, Görke B.
RNA Biol. 2019 Aug;16(8):1055-1065.

Interaction of lipoprotein QseG with sensor kinase QseE in the periplasm controls the phosphorylation state of the two-component system QseE/QseF in Escherichia coli.
Göpel Y, Görke B.
PLoS Genet. 2018 Jul 24;14(7):e1007547.

Carbohydrate Utilization in Bacteria: Making the Most Out of Sugars with the Help of Small Regulatory RNAs.
Durica-Mitic S, Göpel Y, Görke B.
Microbiol Spectr. 2018 Mar;6(2).

Structural insights into RapZ-mediated regulation of bacterial amino-sugar metabolism.
Gonzalez GM, Durica-Mitic S, Hardwick SW, Moncrieffe MC, Resch M, Neumann P, Ficner R, Görke B, Luisi BF.
Nucleic Acids Res. 2017 Oct 13;45(18):10845-10860.

Non-canonical activation of histidine kinase KdpD by phosphotransferase protein PtsN through interaction with the transmitter domain.
Mörk-Mörkenstein M, Heermann R, Göpel Y, Jung K, Görke B.
Mol Microbiol. 2017 Oct;106(1):54-73.

Synthesis and antimicrobial activity of 6-sulfo-6-deoxy-D-glucosamine and its derivatives.
Skarbek K, Gabriel I, Szweda P, Wojciechowski M, Khan MA, Görke B, Milewski S, Milewska MJ.
Carbohydr Res. 2017 Aug 7;448:79-87.

Roles of Regulatory RNAs for Antibiotic Resistance in Bacteria and Their Potential Value as Novel Drug Targets.
Dersch P, Khan MA, Mühlen S, Görke B.
Front Microbiol. 2017 May 5;8:803.

Two Small RNAs Conserved in Enterobacteriaceae Provide Intrinsic Resistance to Antibiotics Targeting the Cell Wall Biosynthesis Enzyme Glucosamine-6-Phosphate Synthase.
Khan MA, Göpel Y, Milewski S, Görke B.
Front Microbiol. 2016 Jun 15;7:908.

Domain swapping between homologous bacterial small RNAs dissects processing and Hfq binding determinants and uncovers an aptamer for conditional RNase E cleavage.
Göpel Y, Khan MA, Görke B.
Nucleic Acids Res. 2016 Jan 29;44(2):824-37.

Cross-Talk between the Canonical and the Nitrogen-Related Phosphotransferase Systems Modulates Synthesis of the KdpFABC Potassium Transporter in Escherichia coli.
Lüttmann D, Göpel Y, Görke B.
J Mol Microbiol Biotechnol. 2015;25(2-3):168-77.